Download Biotechnology Annual Review, Vol. 1 by M. Raafat El-Gewely PDF

By M. Raafat El-Gewely

The Biotechnology Annual evaluate covers some of the advancements in biotechnology within the kind of finished, illustrated and good referenced stories. With the growth of the sphere of biotechnology, coupled with the enormous raise within the variety of new journals reporting contemporary leads to this box, the necessity for a ebook that's continually delivering studies is pressing. for that reason, each one quantity of the Biotechnology Annual evaluate could have a few stories overlaying diverse elements of biotechnology. Reviewed issues will comprise biotechnology purposes in medication, agriculture, marine biology, undefined, bioremedation and the surroundings. primary difficulties facing bettering the technical wisdom encountering biotechnology usage whatever the box of software could be rather emphasised. This sequence might help either scholars and lecturers, researchers in addition to directors to stay an expert on all suitable matters in biotechnology.

Proposals for contributions and/or feedback for subject matters for destiny volumes during this sequence might be despatched to the Editor: professor M.R. El-Gewely division of Biotechnology collage of Tromslø IMB, MH-Bygget N-9037 Tromsø Norway Tel: (+47) seventy seven 644000 Fax: (+47) seventy seven 645350

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Ex vivo gene therapy. In this strategy, tissues such as lymphocytes, hepatocytes, tumor cells, fibroblasts or bone marrow cells are removed from the patient by surgical biopsy and the required genes are introduced into the cells after proper cell isolation and growth in culture. The genetically manipulated cells are then re-implanted in the body of the patient for gene expression or over-expression for the required gene product. Target cells for gene therapy should be readily removable from the patient for in vitro manipulation, they should have the capacity to divide and grow indefinitely and cells containing the target gene should stably continue to express the introduced gene permanently.

Glaxo GIMB molecular microbiology scientist sampling a fermenter during a recombinant mammalian cell culture process. The recombinant protein resulting from such a culture is used for biochemical and biological studies and eventually for drug screening. Courtesy of Glaxo, Greenford. 39 Table 1. Examples of recombinant proteins and vaccines on the market Protein Commercial name Company Use Alteplase (t-PA) Activase Genentech Epoetin-a Epogen Amgen Epoetin-a Procrit Ortho Biotec Factor VIII G-CSF Kognate Neupogen Milesla Bayer Co.

Using experimental animals the volume of the injected material appears to be critical for increased expression of reported genes [236]. Viral transfer methods. Several viral based vectors are used to transform animal cells with high efficiency, such as herpes simplex, retroviral and adenoviral based vectors [ 1631. Vectors based on the adeno-associated virus (AAV) which is non-pathogenic to humans, are also being tested for their potential in gene therapy [237,238]. Viral methods have the potential to be used both for the ex vivo and in vivo gene therapy experiments.

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