Biotechnology

Download Biotechnology of Crucifers by Surinder Kumar Gupta Ph.D. (auth.), Surinder Kumar Gupta PDF

By Surinder Kumar Gupta Ph.D. (auth.), Surinder Kumar Gupta (eds.)

Despite the hot advances made within the development of crucifer vegetation utilizing traditional breeding options, the yield degrees and the oil and meal caliber couldn't be more desirable as anticipated. the certainty of genetic fabric (DNA/RNA) and its manipulation by means of scientists has supplied the chance to enhance crucifers through expanding its range past traditional genetic barriers. the applying of the biotechnological concepts may have significant affects in methods: first, it offers a few techniques/methods for effective choice for favorable versions and moment, it provides a chance to make use of alien version on hand within the crucifers through the use of the radical thoughts of biotechnology to enhance excessive yielding kinds with stable dietary caliber, having resistance to insect, pest, and disorder resistance.

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2009). These various factors affecting microspore embryogenesis have been reviewed by Xu et al. (2007) and Pratap et al. (2009). 6 Doubling Chromosomes The chromosomes are usually unpaired in hybrids of distant hybridization, thereby causing the hybrids sterile. Intergeneric hybridization followed by chromosome doubling of sterile hybrids produce allopolyploids, which might be fertile (Momotaz et al. 1998). Chen et al. (2011) reported that the trigenomic allohexaploids could be obtained via crossing tetraploid × diploid species to produce ABC triploids, followed by chromosome doubling.

In the other hand, 70–90 % of regenerated plants from microspore-derived embryoids are haploid from rapeseed microspore culture (Charne et al. 1988; Chen and Beversdorf 1992). To develop the DH lines, the chromosome doubling technique is needed. Colchicine, which is an anti-microtubule agent to inhibit spindle formation during mitosis in actively-dividing cells, is the most widely-used chemical to double the chromosome (Xu et al. 2007). Chromosome doubling of sterile haploid plantlets is usually achieved by culturing plantlets in colchicine-containing medium in the greenhouse (Mathias and Röbbelen 1991), or soaking roots or whole plants in a colchicine solution (Fletcher et al.

2 Molecular Cytogenetics 19 Notable successes in using fluorescent in situ hybridisation to investigate meiosis in the Brassicaceae include the discovery of a major gene affecting the number of crossovers during meiosis in B. napus (Jenczewski et al. 2003), the elucidation of mechanisms behind stable polyploid formation in Arabidopsis (Comai et al. 2003) and the development of an Arabidopsis thaliana karyotype (Armstrong and Jones 2003). 5 BAC-FISH and Fluorescently Labelled Antibodies The field of molecular cytogenetics is still expanding.

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